Technologies

The massive parallel sequencing or Next Generation Sequencing (NGS) is an innovative and highly versatile technology that allows the parallel sequencing of millions DNA fragments: it allows to perform multiple genes analysis, with a higher diagnostic performance than the traditional sequencing. It drastically reduce genetic analysis times. Microgenomics laboratory uses NGS technology in different specialistic fields: discover the technique’s […]

The array-based Comparative Genomic Hybridization (CGH-array), known as molecular karyotype, is a revolutionary method to investigate the genome. It was developed to identify DNA anomalies known as Copy Number Variations (CNVs), not detectable by other conventional cytogenetic techniques. Copy Number Variations are due to the loss of genomic portions (deletions) or to the presence of […]

A new array technology, known as CGH+SNP array has been recently introduced in the field of molecular cytogenetics. This technology is able to detect not only copy number changes but also uniparental disomies (UPD) and loss of heterozygosity (LOH). Uniparental disomy (UPD) occurs when a person receives two copies of a chromosome or of part […]

Microgenomics laboratory performs genomic investigation by using several array platforms. Every platform has specific features for the prenatal or postnatal field. Array platforms used in our laboratory are: The MicrogenChip arrays have been specifically created by Microgenomics genetic laboratory for diagnostic applications of array-CGH, The genome-wide array platforms, which can be used for different purposes. […]

Quantitative PCR (also called Q-PCR or Real-Time Quantitative PCR) is a technique that amplifies a specific genomic region by monitoring in real time every step of the amplification reaction. This technique is used to establish the number of copies of a particular DNA segment. It allows us to identify deletions or duplications in a specific genomic region. The quantitative […]